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Bleomycin Sulfate

产品编号 T6116Cas号 9041-93-4
别名 Blenoxane, 硫酸博莱霉素, NSC125066, 硫酸博来霉素

Bleomycin Sulfate (Blenoxane) 是一种糖肽抗生素,一种 DNA 合成抑制剂。Bleomycin Sulfate 可以引起 DNA 链断裂而不引起 RNA 链断裂。Bleomycin Sulfate 具有抗肿瘤活性。

Bleomycin Sulfate

Bleomycin Sulfate

纯度: ≥98%
产品编号 T6116 别名 Blenoxane, 硫酸博莱霉素, NSC125066, 硫酸博来霉素Cas号 9041-93-4

Bleomycin Sulfate (Blenoxane) 是一种糖肽抗生素,一种 DNA 合成抑制剂。Bleomycin Sulfate 可以引起 DNA 链断裂而不引起 RNA 链断裂。Bleomycin Sulfate 具有抗肿瘤活性。

规格价格库存数量
1 mg¥ 287现货
2 mg¥ 397现货
5 mg¥ 672现货
10 mg¥ 996现货
25 mg¥ 2,260现货
50 mg¥ 2,750现货
100 mg¥ 4,090现货
200 mg¥ 5,830现货
1 mL x 10 mM (in DMSO)¥ 1,3805日内发货
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纯度:≥98%
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产品介绍

生物活性
产品描述
Bleomycin Sulfate (Blenoxane) is a glycopeptide antibiotic, an inhibitor of DNA synthesis. Bleomycin Sulfate causes DNA strand breaks but not RNA strand breaks. Bleomycin Sulfate has antitumor activity.
靶点活性
UT-SCC-19A cells:4 nM
体外活性
方法:人胰腺细胞 (PCCs) AsPC-1 和 MIA PaCa-2 用 Bleomycin Sulfate (0.1-100 µM) 处理 24-72 h,使用 MTT 方法检测细胞生长抑制情况。
结果:AsPC-1 细胞对 Bleomycin Sulfate 无反应。Bleomycin Sulfate 显著抑制了 MIA-PaCa-2 的细胞生长,在处理 24/48/72 h 时的 IC50 分别为 5.9/6.4/2.6 μM。[1]
方法:间充质干细胞 MSCs 用 Bleomycin Sulfate (1800 ng/mL) 处理 24-96 h,使用 Flow Cytometry 方法检测 sub-G1 和 caspase-3。
结果:Bleomycin Sulfate 诱导 sub-G1 和 caspase-3 的激活上调,表明 Bleomycin Sulfate 诱导细胞凋亡。[2]
方法:正常大鼠肺泡巨噬细胞用 Bleomycin Sulfate (0.01-1 μg/mL) 孵育 18 h,使用 MDGF Assay 测定其水平。
结果:Bleomycin Sulfate 刺激巨噬细胞产生成纤维细胞生长因子 (MDGF)。[3]
体内活性
方法:为检测体内抗肿瘤活性,将 Bleomycin Sulfate (20 mg/kg) 腹腔注射给携带小鼠结直肠癌瘤肿瘤 CT26 shControl 或 shCRT 的 BALB/c 小鼠,每两天一次,给药五次。
结果:Bleomycin Sulfate 延迟了对照 shRNA 转染的 CT26 细胞的生长,但对 CRT shRNA 转染 CT26 细胞没有影响。[4]
方法:为研究博来霉素诱导的小鼠肺纤维化的时间过程,将 Bleomycin Sulfate (0.06 mg/只 in 0.9% saline) 单次气管内滴注 (IT) 给 C57Bl/6J 小鼠。
结果:Bleomycin Sulfate 诱导小鼠肺纤维化。该模型中评估肺纤维化的最合适的时间点是在 IT 滴注 Bleomycin Sulfate 后 14 天,这是基于观察到的动物在 14 天时出现广泛的纤维化,但纤维化反应的变异性较小,死亡率低于21天。[5]
细胞实验
ADIPO-P2 cells are grown in D-MEM high glucose medium supplemented with 20% fetal calf serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) at 37 °C and 5% CO2 atmosphere. Cells are cultured as monolayer in TC25 Corning flasks containing 1.5 × 105 cells/mL. For each experiment, two flasks are set up, one for the control and one for the treated culture. During the log phase of growth ADIPO-P2 cells are treated with a 30 minutes pulse of 2.5 μg/mL of Bleomycin sulfate. Control cultures are set up in parallel but not exposed to Bleomycin sulfate. Time of exposure and concentration of Bleomycin sulfate are chosen according to previous studies carried out in our laboratory with mammalian cells exposed to Bleomycin sulfate. At the end of the pulse treatment with Bleomycin sulfate, the cells are washed twice with Hank's balanced salt solution and kept in culture with fresh culture medium until harvesting. Cells are continuously maintained in culture during 5 passages or subcultures after treatment. Subcultivation is carried out whenever the cultures became confluent (approximately 4 × 105 cells/mL of culture medium). To estimate cell growth, at the time of subcultivation cells are collected by trypsinization, an aliquot of about 200 μL stained with 0.4% trypan blue, and the number of viable cells is determined. Cells are then suspended in fresh culture medium and dispensed into new culture flasks containing 1 × 105 cells/mL to continue growing. The rest of the cells is discarded or dispensed in another flask for cytogenetic analysis, which is performed at 18 hours and 10 days after the end of treatments. To analyze chromosomal aberrations, colchicine (0.1 μg/mL) is added to cell cultures during the last 3 hours of culture. Chromosome preparations are made following standard procedures. After harvesting, cells are hypotonically shocked, fixed in methanol:acetic acid (3:1), spread onto glass slides and processed for PNA-FISH.Two independent experiments are carried out. (Only for Reference)
别名Blenoxane, 硫酸博莱霉素, NSC125066, 硫酸博来霉素
化学信息
分子量1523 (average)
CAS No.9041-93-4
储存&溶解度
存储keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度信息
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
H2O: 92 mg/mL (60.8 mM)
DMSO: 93 mg/mL (61.5 mM)
溶液配制表
H2O/DMSO
1mg5mg10mg50mg
1 mMNaN mLNaN mLNaN mLNaN mL
5 mMNaN mLNaN mLNaN mLNaN mL
10 mMNaN mLNaN mLNaN mLNaN mL
20 mMNaN mLNaN mLNaN mLNaN mL
50 mMNaN mLNaN mLNaN mLNaN mL

计算器

  • 摩尔浓度 计算器
  • 稀释 计算器
  • 配液 计算器
  • 分子量 计算器

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法:
TargetMol | Animal experiments比如您的给药剂量是 10 mg/kg ,每只动物体重 20 g ,给药体积 100 μLTargetMol | Animal experiments 一共给药动物 10 只 ,您使用的配方为 5% TargetMol | reagent DMSO+ 30%PEG300+ 5%Tween 80 + 60% ddH2O. 那么您的工作液浓度为 2 mg/mL
母液配置方法: 2 mg 药物溶于 50 μLDMSOTargetMol | reagent ( 母液浓度为 40 mg/mL ), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:50μLDMSOTargetMol | reagent 母液,添加 300 μLPEG300TargetMol | reagent 混匀澄清,再加 50μLTween 80, 混匀澄清,再加 600μLddH2OTargetMol | reagent 混匀澄清

以上为“体内实验配液计算器”的使用方法举例,并不是具体某个化合物的推荐配制方式,请根据您的实验动物和给药方式选择适当的溶解方案。

1 请输入动物实验的基本信息
mg/kg
g
μL
2 请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
%Tween 80
%ddH2O

剂量转换

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